Supplementary Figure 17: Antigen-specific serum antibody and SBA titers induced by MCV4 and MPSV4 in mice in vivo.
C57BL/6 mice were immunized sub–cutaneously with one human dose (a-c, n=4-9) or two doses (d, n=5) of MPSV4 (blue) or MCV4 (red) or left untreated. (a) Mice were bled after 7, 14, and 28 days and serum IgM, IgG and IgA levels against meningococcal serogroup C were measured. (b) Serum IgG against serogroups A, Y and W-135 were measured at day 28 and (c) Serum Bactericidal Activity (SBA) titers against serogroups A and/or C were measured after 28 days. (d) Mice were boosted with a 2nd dose at day 30. Each gray line represents an individual animal. * p<0.05, ** p<0.01, Mann-Whitney test.
