Supplementary Figure 1: Antibody responses induced by meningococcal vaccines.

(a) ELISPOT captured day 7 IgG antibody-secreting cells (ASCs) in a subset of vaccinees. Plates were coated with goat anti human IgG antibody, MCV4, DT or MPSV4 and detected with anti human IgG biotin and streptavidin HRP. (b) Two representative subjects are shown. On the left, 1X corresponds to 0.17 million PBMCs. DT = Diphtheria Toxoid. (c) Production of IgG is a robust indicator of antibody response. Day 30 IgG correlates well with data from day 180 and 2 years. Data are shown for both vaccines. The day 30/0 IgG data (serogroups A plus C) were thus used for the later analysis of antibody correlation.