Supplementary Figure 3: Aire and MBD1-VP16 share many target genes. | Nature Immunology

Supplementary Figure 3: Aire and MBD1-VP16 share many target genes.

From: The transcriptional regulator Aire coopts the repressive ATF7ip-MBD1 complex for the induction of immunotolerance

Supplementary Figure 3

HEK 293 cells were transfected in triplicate with Flag only (Vector), Aire-Flag (Aire), or Flag-MBD1-VP16 (MBD1-VP16) and microarray analysis was performed on isolated RNA. (a) Comparison of effects of Aire and MBD1-VP16 on transcript levels (each dot represents a single gene). (b) qPCR confirmation of microarray analysis on three additional transcripts induced by both Aire and MBD1-VP16 as shown in (a). Data are representative of three experiments (a,b). Fold induction is calculated relative vector only.

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