Supplementary Figure 4: Ectopic expression of the SCN4B and SCN5A voltage-gated sodium channel subunits confers self-reactivity to CD4+ T cells in proportion to CD5 expression. | Nature Immunology

Supplementary Figure 4: Ectopic expression of the SCN4B and SCN5A voltage-gated sodium channel subunits confers self-reactivity to CD4+ T cells in proportion to CD5 expression.

From: Intrinsic CD4+ T cell sensitivity and response to a pathogen are set and sustained by avidity for thymic and peripheral complexes of self peptide and MHC

Supplementary Figure 4

(a) Gating strategy for the experiments, showing identification of transfected VGSC+ T cells. (b) CD69 upregulation response of VGSC+ LLO56, LLO118 and B6 CD4+ T cells when cultured with or without B6 APCs, or with APCs pretreated with anti I-Ab. Representative primary data (left) and compiled data (right) are presented. (c) Comparison of CD69 responses of untransfected (SCN5A-SCN4B-), singly-transfected (SCN5A-SCN4B+), and doubly-transfected VGSC+ T cells (SCN5A+SCN4B+), in the presence of B6 APCs (without I-Ab blockade). Bars depict means ± SEM. For cells cultured with and without APCs, results from eight cultures over three experiments (LLO56 and LLO118), or six cultures over two experiments (B6 CD4+) were compiled for the graph in panel (b); for blocking studies with anti-I-Ab, 3 or 4 cultures over two experiments were compiled. Statistical analysis was done using a two-tailed Mann-Whitney test. *P < 0.001.

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