Supplementary Figure 6: Loss of the IL-2 responses of LLO56 and LLO118 T cells in adoptive-transfer experiments tracks with deprivation of self peptide–MHC class II. | Nature Immunology

Supplementary Figure 6: Loss of the IL-2 responses of LLO56 and LLO118 T cells in adoptive-transfer experiments tracks with deprivation of self peptide–MHC class II.

From: Intrinsic CD4+ T cell sensitivity and response to a pathogen are set and sustained by avidity for thymic and peripheral complexes of self peptide and MHC

Supplementary Figure 6

(a) Cell surface phenotype of LLO56 and LLO118 T cells following 4-day transfer to B6 or MHC II-deficient recipients, representative of at least three experiments. (b) Ex vivo analysis of PMA + ionomycin-induced IL-2 responses of LLO56 and LLO118 T cells following 4-day transfer to TCR Cα-deficient (LLO56 n = 8, LLO118 n = 6) or H-2M-deficient (LLO56 n = 11, LLO118 n = 10) recipients. Data are compilations of four experiments. (c) Ex vivo analysis of PMA + ionomycin-induced IL-2 responses of freshly isolated LLO56 T cells (n = 4) or LLO56 T cells transferred to H-2M-deficient recipients for the indicated periods of time (Day 1 n = 8, Day 2 n = 6, Day 4 n = 11). (d) Schematic of experiment testing effect of self-pMHC withdrawal on LLO56 and LLO118 T cell response to Listeria in vivo. For (b) and (c), each data point comprising the bar graphs is the % IL-2+ LLO T cells from a single recipient. Bar graphs depict means ± SEM, with statistical analyses done using unpaired two-tailed Student's t tests. NS, not significant and *P < 0.0001.

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