Supplementary Figure 2: Validation of LLO56 and LLO118 signaling results. | Nature Immunology

Supplementary Figure 2: Validation of LLO56 and LLO118 signaling results.

From: Intrinsic CD4+ T cell sensitivity and response to a pathogen are set and sustained by avidity for thymic and peripheral complexes of self peptide and MHC

Supplementary Figure 2

(a) Immunoblot analysis of ERK phosphorylation kinetics of PMA-stimulated LLO56 and LLO118 CD4+ T cells, representative of two experiments. (b) Confirmation of p21 band in 4G10 blots of unstimulated CD4+ whole cell lysates as phospho-TCRζ, using rabbit polyclonal anti-ζ serum 777. 4G10 and anti-ζ serum staining was performed on the same blot. LLO56 was used for this validation as it gave the most easily detectable p21 band. Data are representative of at least three experiments.

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