Supplementary Figure 3: CD5hi B6 CD4+ and CD8+ T cells are more responsive to stimulation than are their CD5lo counterparts. | Nature Immunology

Supplementary Figure 3: CD5hi B6 CD4+ and CD8+ T cells are more responsive to stimulation than are their CD5lo counterparts.

From: Intrinsic CD4+ T cell sensitivity and response to a pathogen are set and sustained by avidity for thymic and peripheral complexes of self peptide and MHC

Supplementary Figure 3

(a) Primary (top) and graphed (bottom) data from IL-2 capture assay analysis of CD4+ and CD8+ T cells stimulated with αCD3 + αCD28. Cells were gated into four equal fractions based on CD5 expression (Q1 through Q4, from lowest to highest CD5 expression). (b) Comparison of CD5 expression on stimulated and unstimulated CD4+ and CD8+ T cells in IL-2 capture (left), intracellular IL-2 (middle), and ERK phosphorylation (right) assays. (c) Overlays of B6 CD4+ and CD8+ T cells pre-sorted into CD5 fractions (Q1 through Q4) by flow cytometry, then analyzed for PMA + ionomycin-induced IL-2 (top) and PMA-induced phospho-ERK responses (3 minute stimulation, bottom). Data are representative of two or three experiments. Bar graphs depict means ± SEM, with statistical analyses done using unpaired two-tailed Student's t tests. *P < 0.01 and **P < 0.001.

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