Supplementary Figure 2: Effects of 2-DG on TLR-induced cytokine production, on binding of GAPDH to cytokine transcripts, on cell death and on activation of Flt3L-DCs. | Nature Immunology

Supplementary Figure 2: Effects of 2-DG on TLR-induced cytokine production, on binding of GAPDH to cytokine transcripts, on cell death and on activation of Flt3L-DCs.

From: TLR-driven early glycolytic reprogramming via the kinases TBK1-IKKɛ supports the anabolic demands of dendritic cell activation

Supplementary Figure 2

(a,c) Nos2-/- GM-DCs were stimulated with LPS in the presence or absence of 2-DG and after 6 h (a) culture supernatants were analyzed for indicated cytokines (c) and cell death was determined by flow cytometry. (a) Bars represent mean ± SEM of triplicate wells. (b) GM-DCs were stimulated with LPS in the presence or absence of 2-DG and after 3 h GAPDH was immunoprecipitated and binding of mRNA of indicated genes to GAPDH was quantified by RT-qPCR. Bars represent mean ±SEM of 3 independent experiments. (d) SIRPα+ and CD24+ DCs were flow cytometry-sorted from Flt3L-BM cultures, stimulated with indicated TLR agonists in the presence or absence of 2-DG and 16 h later expression of indicated surface marker was determined by flow cytometry. Bars represent mean ±SEM of 3 independent experiments. (a,c) One representative experiment out of 2 is shown. *, P < 0.05; **, P < 0.01; ***, P < 0.001

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