Supplementary Figure 3: Glycolytic ATP is not essential for DC activation. | Nature Immunology

Supplementary Figure 3: Glycolytic ATP is not essential for DC activation.

From: TLR-driven early glycolytic reprogramming via the kinases TBK1-IKKɛ supports the anabolic demands of dendritic cell activation

Supplementary Figure 3

(a) Relative ATP levels are shown in GM-DCs at indicated time points following LPS stimulation. Data represent mean ±SEM of duplicate wells. (b) GM-DCs were analyzed as in Fig. 1a in response to treatment with LPS following pretreatment with LDHA inhibitor oxamate. Data represent triplicate wells and are shown as mean ±SEM. (c,d) GM-DCs, pretreated with oxamate, were analyzed for expression of indicated markers (c) or cytokines (d) 6 h after LPS stimulation by flow cytometry, based on surface or intracellular staining, respectively. (c) Expression levels are shown relative to LPS-stimulated DCs. (c) Bars represent at least 3 independent experiments ±SEM. (d) Grey solid and black open histograms represent unstimulated and stimulated cells, respectively. (a,b,d) One representative experiment out of 3 is shown.

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