Supplementary Figure 4: Glycolysis-derived carbon, but not that of glutaminolysis or FA oxidation, is required for DC activation. | Nature Immunology

Supplementary Figure 4: Glycolysis-derived carbon, but not that of glutaminolysis or FA oxidation, is required for DC activation.

From: TLR-driven early glycolytic reprogramming via the kinases TBK1-IKKɛ supports the anabolic demands of dendritic cell activation

Supplementary Figure 4

(a,b) GM-DCs were stimulated with LPS in the presence or absence pyruvate carrier inhibitor α-cyanocinnamate (αCC), CPT1a inhibitor etomoxir (eto) or glutaminase inhibitor 6-Diazo-5-oxo-L-norleucine (DON) and 6 h later expression of indicated surface markers (a) and indicated cytokines (b) was determined by flow cytometry. (a) Data are shown relative to LPS-stimulated DCs and bars represent mean ±SEM of 3 independent experiments. (b) Grey solid and black open histograms represent unstimulated and stimulated cells, respectively. (c) GM-DCs were transduced with retroviral vectors expressing hairpins (hp) or over-expressing constructs (oe) of PDK1 and LDHA or their respective controls. DCs expressing huCD8 and GFP, as reporters for transduction, were analyzed for surface expression of indicated markers after stimulation with medium or LPS for 24 h. (b,c) One representative experiment out of 3 is shown. *, P < 0.01

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