Supplementary Figure 4: Foxp3-bound enhancers are associated with decreased-accessibility to DNase. | Nature Immunology

Supplementary Figure 4: Foxp3-bound enhancers are associated with decreased-accessibility to DNase.

From: Inflammation-induced repression of chromatin bound by the transcription factor Foxp3 in regulatory T cells

Supplementary Figure 4

(a) Foxp3 association with changes in DNase-accessibility is uniform across average locus accessibility. Lines show running average of fold change (y-axis) relative to total accessibility DNase-seq RPM (x-axis). (b) DHSs with decreased accessibility are enriched for Foxp3 binding. This plot represents the same dataset shown in Fig. 4c except for the inclusion of DHSs with q < 0.05 instead of q < 0.01 in Fig. 4c. (c) DHSs that are Foxp3-bound (shown in red) have decreased chromatin accessibility in aTreg vs. Teff cells (y-axis) that are not repressed in rTreg vs. Tn cells (x-axis). (d) Genes near loci with increased DNase accessibility are frequently upregulated in a Foxp3-independent manner. In contrast, many Treg-specific decreases in chromatin accessibility are uniquely associated with Foxp3-dependent decreases in nearby gene expression.

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