Supplementary Figure 5: Foxp3 is associated with activated Treg cell–x96specific increases in H3K27me3.
(a) Activation signals cause changes in H3K27me3 in both aTreg (y-axis) and Teff cell populations (x-axis) relative to their resting counterparts as well as aTreg-specific tri-methylation at a sizable number of loci. (b) Histograms demonstrate that a larger fraction of aTreg specific H3K27me3 blocks (left) have multiple Foxp3 binding sites compared to all H3K27me3 blocks (right) (p < 0.02, KS-test). (c) Decreased expression of Foxp3 target genes with aTreg cell specific increases in H3K27me3. Gene expression changes (x-axis) in aTreg cells compared to Teff (left) and rTreg (right) cells show that Foxp3-bound genes with H3K27me3 (red) have significantly lower expression than those genes that are called as present (black) or are only bound by Foxp3 without increases in H3K27me3 (blue). P values shown are estimated by KS-tests. (d) The H3K27me3 landscape of rTreg cells (y-axis) is largely established in a Foxp3-independent fashion in Teff cells (x-axis). (e) The Pde3b locus contains a Foxp3-bound enhancer that is decreased in accessibility and enriched for H3K27me3.
