Supplementary Figure 6: Increases of H3K27me3 in activated T_reg cells is Foxp3 dependent.

(a) Tracks showing dependence of aTreg H3K27me3 on Foxp3 at the Parp8 (left) and Tcf7 (right) gene loci. Repressed chromatin is present in aTreg cells, but not in any other population, with the most notable being activated Foxp3^GFPKO cells isolated from inflammatory conditions and expressing Foxp3 reporter null allele. (b) H3K27me3 at the Pde3b locus in rTreg cells is dependent on Foxp3. (c, d) aTreg cell-specific H3K27me3 is Foxp3 dependent. Quantification of H3K27me3 changes in rTreg vs. other cell type (c) or aTreg vs. other cell type (d) at Foxp3-bound loci and genome wide. The increase in K27me3 is dependent on Foxp3 (as shown in the comparison to aFoxp3^GFPKO) and only in inflammatory conditions. (e) Treg lineage specific decreases in H3K27me3 marks are not dependent on Foxp3 and can be found in Foxp3^GFPKO cells (left), but not in Teff cells (center) and these genes are upregulated in Treg and Treg precursor Foxp3^GFPKO cells (right).