Supplementary Figure 4: Deficiency in Uhrf1 does not affect the differentiation of colonic T_reg cells.

a-b, In vivo generation of Foxp3⁺ T cells after adoptive transfer of naive CD4⁺ T cells from Cd4^creUhrf1^+/+ and Cd4^creUhrf1^fl/fl mice. A schematic diagram of in vivo differentiation assay of Foxp3⁺ T cells (a). CD4⁺CD44^loCD62^hi naive T cells from Cd4^creUhrf1^+/+ and Cd4^creUhrf1^fl/fl mice were intravenously injected into congenic Foxp3^hCD2 reporter mice. The cLP cells in the recipient mice were analyzed for Foxp3 expression at day 14 after transfer (b). Representative FACS plots are shown. Data are representative of two independent experiments with similar results.