Supplementary Figure 5: Cell populations in LNs of wild-type and CCRL1-deficient mice. | Nature Immunology

Supplementary Figure 5: Cell populations in LNs of wild-type and CCRL1-deficient mice.

From: The atypical chemokine receptor CCRL1 shapes functional CCL21 gradients in lymph nodes

Supplementary Figure 5

(a) LN cellularity in WT (grey box) and CCRL1 ko mice (white box) determined by FACS using counting beads after LN digestion. (b-e) Absolute cell numbers (left) and relative proportions (right) of main immune cell populations. (b) T cells (CD3+), (c) B cells (B220+CD19+) (d) Myeloid cells (CD11c-CD11b+) and (e) DC (CD11c+). Data are from F2 generation 8 week old females; n=6-7 in a-c and n=5 in d-e across three and two litters, respectively. Significance indicated: *, P < 0.05, **, P < 0.01. Box-plot graphs show median values with quartiles and minimum/maximum.

Back to article page