Supplementary Figure 2: CCRL1 expression in the LNs is confined to the gp38^hiLyve-1^lo LEC subset.

Flow cytometry analysis of LECs in lymph nodes from CCRL1-eGFP mice. (a-b) Stromal cells in the live cell gate (a), gated from the CD45^neg, Ter119^neg cells (b). (c) LECs are defined as gp38^high CD31^high cells, BECs as gp38^low CD31^high, FRC as gp38^high CD31^neg, and DN stromal cells are defined as gp38^neg CD31^neg. (d) gp38^high CD31^high LECs analysed for expression of LYVE-1 and eGFP. (e-f) Analysis of the eGFP^neg and eGFP^pos gated LEC subsets for expression of gp38 (e) and Lyve-1 (f). Geometric mean (MFI) and LYVE-1 positive cells in gate L1 are shown. eGFP positive cells in the gp38^high CD31^low, FRCs (g), the gp38^low CD31^high, BECs, (h) the gp38^low CD31^low DN stromal cells (i) and CD45^pos gated cells (j). Representative graphs from inguinal LN from 3 CCRL1-eGFP mice.