Supplementary Figure 5: Rhbdd3 negatively regulates TLR and TNF-α-induced NF-κB pathways in BMDCs but not in BMDMs and MEF. | Nature Immunology

Supplementary Figure 5: Rhbdd3 negatively regulates TLR and TNF-α-induced NF-κB pathways in BMDCs but not in BMDMs and MEF.

From: Rhbdd3 controls autoimmunity by suppressing the production of IL-6 by dendritic cells via K27-linked ubiquitination of the regulator NEMO

Supplementary Figure 5

(a) Immunoblot of NF-κB and MAPK signaling proteins in Rhbdd3+/+ and Rhbdd3–/– BMDCs stimulated with CpG, Poly(I:C) and TNF. (b) EMSA analysis of NF-κB DNA binding ability in nuclear extracts of Rhbdd3+/+ and Rhbdd3–/– BMDCs stimulated with LPS. (c, d) Immunoblot of NF-κB and/or MAPK signaling proteins in Rhbdd3+/+ and Rhbdd3–/– BMDCs and BMDMs (c) and MEFs (d) stimulated with LPS. Data are from one representative of three independent experiments with similar results.

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