Supplementary Figure 6: Rhbdd3 interacts with K27-linked polyubiquitin chains on NEMO. | Nature Immunology

Supplementary Figure 6: Rhbdd3 interacts with K27-linked polyubiquitin chains on NEMO.

From: Rhbdd3 controls autoimmunity by suppressing the production of IL-6 by dendritic cells via K27-linked ubiquitination of the regulator NEMO

Supplementary Figure 6

(a) Immunoblot in HEK293 cell lysates after single and sequential V5 IP. (b) Immunoblot in HEK293 cell lysates after sequential V5 IP as in (a). Numbers below HA-lanes indicate densitometry of HA relative to V5 expression in that same lane. (c) Immunoblot in HEK293 cell lysates after Flag IP. (d) Immunoblot in HEK293 cell lysates after V5 IP. (e) Silver staining of in vitro purified GST proteins. (f) Schematic of IP procedure in Fig. 6f. (g) Immunoblot in HEK293 cell lysates after V5 IP. Data are from one representative of three independent experiments with similar results.

Back to article page