Supplementary Figure 1: Enhanced proliferation and Th1/Th17 differentiation of Rhbdd3–/– CD4+ T cells. | Nature Immunology

Supplementary Figure 1: Enhanced proliferation and Th1/Th17 differentiation of Rhbdd3–/– CD4+ T cells.

From: Rhbdd3 controls autoimmunity by suppressing the production of IL-6 by dendritic cells via K27-linked ubiquitination of the regulator NEMO

Supplementary Figure 1

(a) Appearances of spleens and inguinal lymph nodes (LNs) from 6-month-old Rhbdd3–/– and Rhbdd3+/+ mice (n=3 mice per group). (b) Rhbdd3 mRNA expression in each of the indicated organs and immune cell types were measured by RT-PCR. (c, d) The percentages of CD4+CD25+Foxp3+ Treg in the spleen of Rhbdd3–/– and Rhbdd3+/+ mice were identified by FACS (c) and quantified (d, n=3 mice per group). The dot plots were gated on CD4+ T cells. (e) Proliferation of splenic CD4+ T cells stimulated with α-CD3/28. (f) Production of IFN-γ and IL-17 by MLN (mesenteric LNs) cells, splenocytes or CD4+ T cells stimulated with α-CD3/28. (g, h) Percentages of Th1 and Th17 cells in splenic T cells after α-CD3/CD28 stimulation were analyzed by FACS (g) and quantified (h). Med, Medium. *P<0.05, **P<0.01, ns, not significant (two tailed Student's t-test). Data are from three independent experiments (mean±s.d. of three mice (d) or of technical triplicates (f, h)) or are representative of three independent experiments (a-c, e, g).

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