Supplementary Figure 1: JAK/STAT signaling and the type III interferon pathway.
From: Diverse intracellular pathogens activate type III interferon expression from peroxisomes
(A) STAT1 and (B) JAK2 were knocked down in Huh7 cells. (C) Human Huh7.5 cells were incubated with mouse IFNλ2 or IFNβ, and STAT1 phosphorylation was assessed by western immunoblotting. (D) 293T cells expressing luciferase under the control of an ISRE promoter were incubated with mouse IFNλ2, human IFNλ1 or mouse or human IFNβ. Cells were then assessed for their ability to respond to IFNs by producing luciferase. Error bars represent mean ± SEM of triplicate readings for one experiment representative of 3. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (One-way ANOVA).
