Supplementary Figure 4: TH9 transcription factor analysis in wild-type and IL-9-deficient mice | Nature Immunology

Supplementary Figure 4: TH9 transcription factor analysis in wild-type and IL-9-deficient mice

From: TH9 cells that express the transcription factor PU.1 drive T cell–mediated colitis via IL-9 receptor signaling in intestinal epithelial cells

Supplementary Figure 4

(a) Expression of pro-inflammatory cytokines was analyzed using quantitative RT-PCR. Total mRNA was isolated from the inflamed colon of wild-type and II9-deficient mice (n= 8-21) followed by qPCR analysis. Data were normalized to the house keeping gene 18sRNA. Significant differences are indicated. (b) Expression of T cell transcription factors was analyzed using quantitative RT-PCR. Data were normalized to the house keeping gene 18sRNA. Levels of Gata3, Irf4, Tbet, Foxp3, Rorγt and Spi1 were analyzed in II9 knockout mice as compared to wild-type animals (n= 14-18). (c) Immunofluorescence staining of PU.1 was performed on cryosections from colonic tissue of wildtype and II9 knockout mice. Cell nuclei were counterstained with HOECHST dye. Representative stainings are shown. In addition, double staining with CD4 was performed and significant changes are indicated.

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