Supplementary Figure 6: ChIP-sequencing analysis identifies AP4 and c-Myc target genes in CD8+ T cells.
From: c-Myc-induced transcription factor AP4 is required for host protection mediated by CD8+ T cells
(a-d) Annotation and motif analysis of genomic sequences bound by AP4 (a,b) or c-Myc (c,d) in activated C57BL/6 CD8+ T cells. (e) Examples of AP4 and c-Myc binding to genes that are differently expressed between Tfap4–/– and Tfap4+/+ OT-I T cells in vivo. BedGraph histograms are shown with statistically significant peaks as red rectangles. P < 1 × 10-10 for anti-AP4 ChIP, P < 5 × 10-9 for anti-c-Myc ChIP by Poisson distribution. ChIP-seq data for RNA polymerase II C-terminal domain (polII-CTD) serine phosphorylations (S2P and S5P) are shown as reference to transcribed regions. Data are from one experiment.
