Supplementary Figure 2: Role of CCR2 in the development and phenotype of colonic macrophages.

(a) Representative expression of Ly6C and MHCII expression by total F4/80⁺ CD11b⁺ colonic LP cells from newborn (day 0-1), day 7, day 14 and day 21 Ccr2^+/+ and Ccr2^–/– mice. (b) Absolute numbers of F4/80⁺ CD11b⁺ Ly6C^hiMHCII^-, Ly6C⁺MHCII⁺ and Ly6C^- cells per colon of newborn (day 0-1), day 7, day 14, day 21 and 7-8 week old adult Ccr2^+/+ (filled circles) and Ccr2^–/– mice (open circles). Data pooled from two independent time course experiments with a total of 6 mice (newborn (day 0-1), day 7, 14 and 21 time points) or 9 mice (adult time point). * P<0.05, *** P<0.01, *** P<0.001 (Student’s t-test). (c) Representative MHCII expression by F4/80⁺ CD11b⁺ Ly6C^– cells from newborn (day 0-1), day 7, day 14, day 21 and 7-8 week old adult Ccr2^+/+ (filled histogram) and Ccr2^–/– mice (open histogram). (d) Representative expression of F4/80 and CD11b by live CD45⁺ SiglecF^– Ly6G^– CD11c^lo colonic LP cells (top panels), and Ly6C and MHCII expression by total F4/80⁺ CD11b⁺ cells (bottom panels) from 9-12 month old Ccr2^+/+ and Ccr2^–/– mice. Scatter plots show the absolute numbers of Ly6C^hiMHCII^–, Ly6C⁺MHCII⁺ and Ly6C^– subsets amongst F4/80⁺ CD11b⁺ cells per colon of 9-12 month old Ccr2^+/+ (filled circles) and Ccr2^–/– mice (open circles). Data from one experiment with 5-6 mice per group. *** P<0.001 (Student’s t-test).