Supplementary Figure 3: Enhanced levels of ROS endogenously activate ATM, AMPK and p38 in senescent CD27–CD28– CD4+ T cells. | Nature Immunology

Supplementary Figure 3: Enhanced levels of ROS endogenously activate ATM, AMPK and p38 in senescent CD27–CD28– CD4+ T cells.

From: The kinase p38 activated by the metabolic regulator AMPK and scaffold TAB1 drives the senescence of human T cells

Supplementary Figure 3

(a) Intracellular ROS levels by Dihydroethidium (DHE) in CD27+ CD28+ and CD27- CD28- CD4+ T cells directly ex vivo, as measured by flow cytometry. Data are representative of 4 independent experiments. (b) Representative overlays and (c) pooled phospho-flow data from 3 different donors showing the effect of the ROS scavenger superoxide-dismutase (SOD; 100 U, 60’) on AMPK (Thr172), p38 (Thr180,Tyr182) and ATM (Ser1921) phosphorylation in senescent CD27- CD28- CD4+ T cells. All *p< 0,05, **p<0.01, and ***p< 0.001 values were calculated by a paired Student’s t test. Error bars depict s.e.m

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