Supplementary Figure 2: PARM-1 is decorated with PNAd in mouse lymph nodes. | Nature Immunology

Supplementary Figure 2: PARM-1 is decorated with PNAd in mouse lymph nodes.

From: Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing

Supplementary Figure 2

Representative immunoblot (WB) of Parm1 immunoprecipitate (IP) from mouse lymph node membrane enriched protein probed for Parm1 (green) and MECA-79 (red). * indicates two isoforms of Parm1 proteins, of which the higher molecular weight band is also detected by MECA-79, but in parallel experiments did not bind control rat IgM (not shown). Control IP is IP using anti-Hsp90 antibody, a monoclonal rabbit IgG. Numbers on the left indicate the position of molecular weight standard proteins of the indicated kDa. Data are representative of 3 independent experiments with axillary, brachial and in some instances mesenteric lymph nodes pooled from 2-3 mice in each case.

Back to article page