Supplementary Figure 4: Analysis of M1 and M2 macrophage markers. | Nature Immunology

Supplementary Figure 4: Analysis of M1 and M2 macrophage markers.

From: Perivascular leukocyte clusters are essential for efficient activation of effector T cells in the skin

Supplementary Figure 4

(a) TRITC-conjugated dextran was injected and dermal suspension was prepared 24 h later. CD45+ and TRITC+ cells were further analyzed with CD11b and F4/80 antibodies by flow cytometry. (b) M1 macrophage markers, such as TNF-a, Nos2, and IL-12a, and M2 macrophage markers, such as arginase (Arg)-1, Retnla, and Chi313, were examined in BM-derived M1 and M2 macrophages. Each bar represents the mean + SD (n=3). A.U., arbitrary units. *, P < 0.05. ***P < 0.0001.

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