Supplementary Figure 5: Neutrophils are not essential for CXCL2 expression in DNFB-painted skin. | Nature Immunology

Supplementary Figure 5: Neutrophils are not essential for CXCL2 expression in DNFB-painted skin.

From: Perivascular leukocyte clusters are essential for efficient activation of effector T cells in the skin

Supplementary Figure 5

(a) Relative amount of Il1r1 24 h after with or without DNFB-sensitization (n=5). (b) Relative amount of Cxcl2 in DNFB-painted skin in 1A8- or control IgG-treated mice (n=5, each). (c) FACS plot of DNFB-painted skin prepared from 1A8- or control IgG-treated mice. CD11b+ Gr-1+ neutrophils were significantly depleted with 1A8-treatment. (d) Relative amount of Il1r1 from dermal macrophages cultured with or without IL-1α (n=4, each). (e) RT-PCR analysis of chemokine receptor mRNA expression in BM-derived DCs.

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