Supplementary Figure 2: Subset-specific depletion of cutaneous DCs. | Nature Immunology

Supplementary Figure 2: Subset-specific depletion of cutaneous DCs.

From: Perivascular leukocyte clusters are essential for efficient activation of effector T cells in the skin

Supplementary Figure 2

(a) A schematic representation of our strategy to generate subset-specific cutaneous DC depletion models. To deplete all cutaneous DC subsets, Langerin-DTR mice were transferred with BM cells from CD11c-DTR mice, and DT was injected. To selectively deplete LCs, Langerin-DTR mice were transferred with BM cells from C57BL/6 mice, and DT was injected. To selectively deplete dDCs, C57BL/6 mice were transferred with BM cells from CD11c-DTR mice, and DT was injected. BMT; BM transplantation. (b) FACS plots of each group of mice after DT treatment. In dermis, the percentages in CD45+ cells were indicated. (c) Histological findings of the ear skin after CHS. HE staining of the ears of mice 24 h after challenge with DNFB. Mice were pretreated with or without sensitization, depleted of LCs and/or dDCs, and challenged with DFNB. Scale bar = 100 μm. (d) CHS response was induced on the ear skin, and skin-infiltrating cells were stained and analyzed with TCR beta, CD44, and CD62L antibodies by flow cytometry.

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