Supplementary Figure 3: dDC clusters are formed in response to various stimuli. | Nature Immunology

Supplementary Figure 3: dDC clusters are formed in response to various stimuli.

From: Perivascular leukocyte clusters are essential for efficient activation of effector T cells in the skin

Supplementary Figure 3

(a) The scoring criteria for DC clusters by numbers and diameters of clusters. (b) DC (green) cluster formation 24 h after topical application without (NT) or with acetone, olive oil, 3% TNCB, 2% DNTB, 0.3% DNFB, or Mycobacterium bovis BCG-inoculation (n=4, each). (c) Scores of DC cluster numbers of each group 24 h after each stimuli. (d) DC (green) cluster formation 24 h after topical application without (NT) or with 0.5% DNFB on the back skin and footpad. Scale bar = 100 μm. (e) Mobility of DCs and T cells of the cluster by treatment with anti-LFA-1 treatment. Anti-LFA-1 neutralizing antibody, KBA, was injected intravenously 14 h after elicitation. T cell (red) clustering was dissolved but DC (green) clustering persisted 10 h after KBA-treatment. Scale bar = 100 μm. (f) Score of DC cluster number 24 h after DNFB application with KBA (red) or control IgG (black) treatment (n=5, each).

Back to article page