Supplementary Figure 2: Lack of roquin in T cells does not induce inflammation in skin, intestine and joint.

(a) Lung CD4⁺ T cells from 12–18-week-old WT and Rc3h1^fl/flRc3h2^fl/flCd4-Cre mice were stimulated and stained for CD4 versus IL-4. (b) Lung CD4⁺ T cells from 12–18-week-old WT and Rc3h1^fl/flRc3h2^fl/flCd4-Cre mice were stained for CD4 and RORγt. (c) Lung CD45⁺ cells from 12–18-week-old WT and Rc3h1^fl/flRc3h2^fl/flCd4-Cre mice were stimulated and stained for IL-17A versus CCR6. (a-c) FACS plots are representative of three independent experiments. (d) H&E-stained skin sections from WT and Rc3h1^fl/flRc3h2^fl/flCd4-Cre mice (20x magnification, scale bar represents 100 μm). (e) Quantification of CD3⁺, CD45⁺ and p-STAT3⁺ cells in skin sections. (f) H&E-stained small intestine sections from WT and Rc3h1^fl/flRc3h2^fl/flCd4-Cre mice (20x magnification, scale bar represents 100 μm). (g) Quantification of CD3⁺, CD45⁺ and p-STAT3⁺ cells in small intestine sections. (h) H&E-stained joint sections from WT and Rc3h1^fl/flRc3h2^fl/flCd4-Cre mice (40x magnification, scale bar represents 100 μm). (d, f, h) Representatives of two technical replicates from three 26–30-week-old WT and Rc3h1^fl/flRc3h2^fl/flCd4-Cre mice are shown. (e, g) Results represent mean ± SEM, *, P = 0.0269; NS, not significant (unpaired, two-tailed t test). (i) FACS-analyses of IL-17A versus IFN-γ in PMA and ionomycin-stimulated CD4⁺ T cells from small and large intestine of 12–18-week-old WT and Rc3h1^fl/flRc3h2^fl/flCd4-Cre mice. FACS plots are representatives of four independent experiments.

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