Supplementary Figure 1: Comparison of lung inflammation and mortality in mice with roquin mutations. | Nature Immunology

Supplementary Figure 1: Comparison of lung inflammation and mortality in mice with roquin mutations.

From: Cleavage of roquin and regnase-1 by the paracaspase MALT1 releases their cooperatively repressed targets to promote TH17 differentiation

Supplementary Figure 1

(a) Histological sections of H&E-stained (magnification 20x, scale bar represents 100 μm) and anti-CD3-, anti-MHCII- and anti-Ki67-stained (magnification 40x, scale bar represents 10 μm) lung tissues from 26–30-week-old WT and Rc3h1fl/flRc3h2fl/flCd4-Cre mice. (b) Total numbers of CD45+ cells in lung tissue of 26–30-week-old WT control and Rc3h1fl/flRc3h2fl/flCd4-Cre mice were determined by flow cytometry. (c) Relative numbers of CD3+ cells in lung tissue of 26–30-week-old WT control and Rc3h1fl/flRc3h2fl/flCd4-Cre mice were detected by histology. (d) Relative numbers of MHCII+ cells detected as in c. (e) Relative number of Ki67+ cells detected as in c. (a,c-e) Results represent analyses of 5 mice for each genotype or (b) 8 WT and 7 Rc3h1fl/flRc3h2fl/flCd4-Cre mice. Error bars are mean ± SEM, *, P = 0.0435; **, P = 0.0266; ***, P = 0.0008; ****, P < 0.0001 (unpaired, two-tailed t-test). (f) Kaplan-Meier survival curve of Rc3h1fl/flRc3h2fl/flCd4-Cre mice. (g) Titers of anti-DNA antibodies in sera from 4 WT, 5 Rc3h1fl/flRc3h2fl/flCd4-Cre, and 4 Rc3h1san/san mice as detected by ELISA. (h) Titers of rheumatoid factor detected as in g. (g-h) Error bars are mean ± SD, *, P = 0.0135; **, P = 0.0039; ***, P = 0.0012; ****, P = 0.0005 (One way ANOVA with Tukey’s test).

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