Supplementary Figure 6: Trpv1–/– naive CD4+ T cells have impaired colitogenic properties in an adoptive transfer model.
From: The ion channel TRPV1 regulates the activation and proinflammatory properties of CD4+ T cells
(a) Representative pictures of colons from Rag1–/– recipient mice 4 weeks after adoptive transfer of 3 × 105 wild-type (WT) or Trpv1–/– naive (CD4+CD45RBhiCD25–) T cells or a mixture of 3 × 105 WT naive CD4+ T cells plus 1.5 × 105 WT Treg (CD4+CD45RBloCD25+) cells (WT naive T cells + Treg) as in Figure 7d. (b) Colon length in each experimental group. (c) Microcopy of colon sections from mice as in a, stained with the DNA-binding dye DAPI, Alexa Fluor 546–conjugated anti-CD45 and Alexa Fluor 488–conjugated anti-Claudin-3 antibodies. Scale bar, 50 μm (40× objective). (d) Real-time PCR analysis of several proinflammatory cytokines (top panel) and chemokines (bottom panel) in colon samples. The Treg control group was used as reference and assigned to 1. (mean ± s.e.m. (n = 6-8 mice/group)). NS, not significant; *P < 0.05, **P < 0.01 and ***P < 0.001 (one-way analysis of variance (ANOVA) with post-hoc Bonferroni’s test).
