Supplementary Figure 3: Methods for the analysis of CLPs and intracellular phosphorylated STAT5. | Nature Immunology

Supplementary Figure 3: Methods for the analysis of CLPs and intracellular phosphorylated STAT5.

From: The transcription repressors Bach2 and Bach1 promote B cell development by repressing the myeloid program

Supplementary Figure 3

a. Flow cytometry analysis of CLPs. WT bone marrow cells were analysed for surface expression of Lin–, PI–, IL7Ra+, c-kitmid, Flt3+ and Sca-1mid. The staining reagents are described in online materials and methods. b. Surface marker staining of Lin– cells sorted by MACS. c. The levels of phosphorylated STAT5 (pSTAT5) of CLPs obtained from WT (dotted line) and DD (solid line) mice. The shaded histogram represents the results obtained with an isotype control (gray). High phosphorylation areas (not detected with the isotype control) were gated.

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