Supplementary Figure 6: Anti-PDPN does not block the binding of CLEC-2 or impair DC migration. | Nature Immunology

Supplementary Figure 6: Anti-PDPN does not block the binding of CLEC-2 or impair DC migration.

From: The CLEC-2–podoplanin axis controls the contractility of fibroblastic reticular cells and lymph node microarchitecture

Supplementary Figure 6

(a) Histogram indicating CLEC-2-Fc staining of FRCs that were either untreated, pre-treated with a hamster isotype control antibody, or pre-treated with the 8.1.1 PDPN-specific antibody. Grey line indicates the secondary alone control for the CLEC-2-Fc staining. (b,c) DC numbers (gated on PI−CD45+CD11chi) in LNs of isotype- or -PDPN-treated mice in steady state (b) or after PBS or LPS/OVA administration (c). Data are representative of 4 independent experiments (n=3-4 mice per group per experiment). *P<0.05 (Mann Whitney test).

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