Supplementary Figure 1: PDPN is required for cell adhesion and contraction. | Nature Immunology

Supplementary Figure 1: PDPN is required for cell adhesion and contraction.

From: The CLEC-2–podoplanin axis controls the contractility of fibroblastic reticular cells and lymph node microarchitecture

Supplementary Figure 1

(a) Representative histograms of FRC surface markers on wild-type and Pdpn−/− FRCs. (b) Graph depicting the area covered by FRCs as they spread on collagen at different time points after plating. (c) Graph representing the absorbance of crystal violet, which was used to stain FRCs adhering to collagen-coated wells at different time points after plating. (d-f). Wild-type FRCs were seeded into 3D collagen gels and then treated with the ROCK inhibitor Y27632. FRC morphology index (d) and the length (e) and number (f) of protrusions were measured. Data are representative of 3 independent experiments (mean±s.d., n>50 cells per condition per experiment). (g) Representative image of YAP staining in wild-type and Pdpn−/− FRCs. Data are representative of 3 independent experiments. NS, not significant *P<0.05; ** P<0.01; *** P<0.001; **** P<0.0001 (Mann Whitney test).

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