Supplementary Figure 5: Skewed CD5 distribution on naive LM-B8R–H-2K^b–specific CD8⁺ T cell precursors.

The gates demarcating the lower and upper 20% for CD5 expression on bulk CD44^lo CD8⁺ T cells cells were applied to CD44^lo B8R/K^b tetramer stained CD8⁺ T cells from the same sample. The data indicate what percentage of B8R/K^b specific cells are contained in the gates used for sorting CD5^hi and CD5^lo cells. Values above 20% indicate the B8R/K^b specific naïve CD8⁺ T cells was overrepresented in that sorted population, values less than 20% indicate B8R/K^b specific population were underrepresented in that group. Data are cumulative from 5 separate experiments (n=17) and are shown as mean ± SEM. Median values were used to calculate the fold-change from the bulk population (i.e. by how much the B8R/K^b-specific population is under- or overrepresented in that population).