Supplementary Figure 1: Phenotypic characteristics of naive CD5lo and CD5hi CD8+ T cells in wild-type and Il15−/− mice. | Nature Immunology

Supplementary Figure 1: Phenotypic characteristics of naive CD5lo and CD5hi CD8+ T cells in wild-type and Il15−/− mice.

From: The TCR's sensitivity to self peptide–MHC dictates the ability of naive CD8+ T cells to respond to foreign antigens

Supplementary Figure 1

Peripheral lymphocytes were isolated from WT (a and c) or IL-15-/- (b and c) mice. Naive (CD44lo) CD8 T cells were gated on the lower (red) and upper (blue) 20% of CD5 expression and compared to memory phenotype (MP) cells (CD44hiCD122hi, gray shaded). Flow plots are representative of 2 separate experiments. (d) CD5hi and CD5lo naïve CD8 T cells were sorted and transferred into normal hosts (as in Fig 1c). The graph shows the percentage of each population that acquired CD44hi (memory)-phenotype, presented as mean ± SEM). Data in (a,c) are representative of 4 experiments and those in (b) 2 experiments. * indicates p<0.05.

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