Supplementary Figure 3: Analysis of TFH and GC B cells.
From: Treg cells require the phosphatase PTEN to restrain TH1 and TFH cell responses
(a) Flow cytometry of CXCR5 and PD-1 expression (gated on CD4+TCRβ+ cells) in the MLNs of WT and Ptenfl/flFoxp3-Cre mice. (b,c) Flow cytometry of CXCR5 and ICOS (b), and CXCR5 and Bcl6 (c) expression (gated on CD4+TCRβ+ cells) in the spleen and MLNs of WT and Ptenfl/flFoxp3-Cre mice. (d) Flow cytometry of GL7 and CD95 expression (gated on CD19+ cells) in the MLNs of WT and Ptenfl/flFoxp3-Cre mice. (e,f) Analysis of TFH (e) and GC B cells (f) in the spleen of mice immunized with NP-OVA 7 d previously. Data are representative of three independent experiments (a-d) and one experiment (e,f; n = 4 WT, 4 Ptenfl/flFoxp3-Cre mice). *P < 0.05. Data are mean ± s.e.m. Numbers indicate percentage of cells in gates.
