Supplementary Figure 5: Analysis of Treg cells in Ptenfl/flFoxp3-Cre Ifng−/− mice and Ptenfl/flFoxp3-Cre Treg cell phenotypes under steady state and after adoptive transfer.
From: Treg cells require the phosphatase PTEN to restrain TH1 and TFH cell responses
(a) Flow cytometry analysis of Treg cells in WT, Ptenfl/flFoxp3-Cre, Ifng−/− and Ptenfl/flFoxp3-Cre Ifng−/− mice. (b) Expression of CXCR3, CXCR5, T-bet, Bcl6, IRF4 and p-STAT3 in Treg cells from the spleen of WT and Ptenfl/flFoxp3-Cre mice; numbers above graphs indicate MFI. (c) Analysis of CXCR3 and T-bet expression in Treg cells in the spleen of WT, Ptenfl/flFoxp3-Cre, Ifng−/− and Ptenfl/flFoxp3-Cre Ifng−/− mice. (d) Analysis of Foxp3 (left) and CD25 (right) expression in PLNs and MLNs of CD45.1+ recipients after transferring WT and Ptenfl/flFoxp3-Cre Treg (CD45.2+) cells; numbers above graphs indicate MFI. Data are representative of at two independent experiments (a-d). Numbers indicate percentage of cells in gates.
