Supplementary Figure 4: iILC2 cells produce IL-13 and become nILC2-like cells during N. brasiliensis infection.

(a) 4C13R mice were infected with N. brasiliensis for various days and lung leukocytes were isolated and analyzed by flow cytometry for lineage, Thy1, KLRG1, AmCyan and DsRed expression. nILC2s were gated on Lin^–Thy1^hiKLRG1^int (red) and iILC2s were gated on Lin^–Thy1^low KLRG1^hi (blue). (b) 4 ×10⁴ nILC2s sorted from naïve CD45.1 Rag1^–/– mice or 20 ×10⁴ iILC2s sorted from IL-25-treated CD45.1 Rag1^–/– mice were transferred into Rag2^–/–Il2rg^–/– mice, which were infected with N. brasiliensis. 14 days later, transferred cells in the lung were analyzed by flow cytometry for expression of CD45.1, KLRG1, ST2 and Thy1. Data are representative of two independent experiments in a, b (n=3 mice for each group in each experiment of a, n=2 recipient mice in each experiment of b).