Supplementary Figure 5: Both IL-17-producing and non–IL-17-producing iILC2 cells can develop into IL-17–IL-13 double producers in vitro.
iILC2s, from IL-25-treated IL-17-RFP mice, were stimulated with PMA plus ionomycin and then were divided into RFP+ and RFP– populations through cell sorting. The purified populations were cultured in either “TH2” conditions or “TH17” conditions for 7 days. IL-13 and IL-17 production was measured by intracellular staining after 6-hour stimulation with PMA plus ionomycin. Data are representative of two independent experiments (n=3 cell culture wells for each condition in each experiment).
