Supplementary Figure 6: nILC2 cells have less ability than iILC2 cells to develop into IL-17 producers.

nILC2s (sorted from naïve Rag2–/– mice) and iILC2s (sorted from IL-25-treated Rag2–/– mice) were cultured in various conditions as indicated. 7 days after culture, cells were stimulated with PMA plus ionomycin for 6 hours and the production of IL-13 and IL-17 were determined by intracellular cytokine staining. Data are representative of two independent experiments (n=2 cell culture wells for each condition in each experiment).