Supplementary Figure 5: Similar TEM cell differentiation by Gpr15-het and KO cells.
From: Role and species-specific expression of colon T cell homing receptor GPR15 in colitis
Similar TEM cell differentiation by Gpr15-het and KO cells. (a) Naive CD45RBhi CD4+ T cells from Gpr15-KO (CD45.2) or het (CD45.1, CD45.2) mice were co-injected into Rag2-/- recipients in equal proportion. After two weeks, Gpr15-het- and KO-derived CD4+ T cells display similar frequencies of IFN-γ and IL-17 expression. Representative flow cytometry plots (b), non-normalized (c) and normalized data (d) are shown. (e) Naive CD4+ T cells from Gpr15-het or KO mice were injected into Rag2-/- recipients, and GPR15 (GFP+) CD4+ T cells were sorted from recipient colon or MLN after two weeks. (f) Expression of IFN-γ, IL-17, Foxp3 and Ki-67 by sorted GFP+ CD4+ T cells from pooled colon or MLN. Results of a single experiment performed are shown for comparison with the cytokine expression of total het- and KO-derived effector cells in (3b).
