Supplementary Figure 6: Minimal Foxp3 mRNA expression by human colon GPR15+ memory CD4+ T cells, and comparison of GPR15 expression by resting and activated human Treg cells.
From: Role and species-specific expression of colon T cell homing receptor GPR15 in colitis
Minimal Foxp3 mRNA expression by human colon GPR15+ memory CD4+ T cells, and comparison of GPR15 expression by resting and activated human Treg cells. (a) Expression of GPR15, Foxp3 and II2ra in sorted human colon cells (as indicated) from 1 UC and 2 non-UC samples was determined by Realtime RT-PCR and normalized to GAPDH. n = 3, mean + s.e.m., *P < 0.05, unpaired Student’s t-test. (b) GPR15 staining of rested and activated human colon Treg cells. Human colonic lymphocytes were stained with polyclonal anti-GPR15 antibody pre or post activation with PMA and ionomycin at 37 °C. Cells are gated on CD45RO+ CD4+ T cells. (c,d) Colon T cell GPR15 expression in Crohn’s disease. Lymphocytes isolated from resectioned colon of 2 Crohn’s patients were activated and incubated with antibodies to GPR15 (or rabbit IgG isotype), Foxp3 and cytokines. Cells are gated on memory CD4+ T cells (CD4+, CD45RO+).
