Supplementary Figure 2: GPR15 mediates accumulation of TEM and Treg cells in the mouse colon.
From: Role and species-specific expression of colon T cell homing receptor GPR15 in colitis
GPR15 mediates accumulation of TEM and Treg cells in the mouse colon. (a) Gpr15-KO (CD45.2) or het (CD45.2) bone marrow cells were combined with Gpr15-het (CD45.1) cells and transferred into irradiated hosts (CD45.1 × CD45.2 F1) to generate experimental (Exp) or control (Ctrl) chimeras, respectively. (b) Frequency of Gpr15-het and Gpr15-KO cells among CD4+ T subsets in experimental chimeras. (c) Splenocytes from DO11.10 Gpr15-het or DO11.10 Gpr15-KO were i.v. injected into Balb/c (Thy-1.1) mice. After 24 h, recipients were treated intra-rectally with OVA and CT. Accumulation of OVA-specific CD4+ T cells in tissues was assessed 5 days later. (d) Cells are gated on Gpr15-het or KO derived antigen-specific (DO11+) memory CD4+ T cells. (e) CFSE-labeled splenocytes from Gpr15-het (Thy-1.1, Thy-1.2) and KO (Thy-1.2) DO11 mice were co-injected i.v. in equal proportion into congenic non-DO11 hosts (Thy-1.1) on day 0. Recipients were treated with OVA and CT per rectum on day 1, FTY720 or PBS i.p. on day 3, and tissues were collected on day 5.
