Supplementary Figure 5: Unaltered expression of molecules with a presumed role in T_reg cell–mediated suppression in the absence of CK2β.

(a) Mean fluorescence intensity (MFI) of the indicated molecules known to be involved in T_reg cell-mediated suppression in CD4⁺Foxp3⁺ T_reg cells of Csnk2b^fl/fl and Csnk2b^fl/flFoxp3-Cre mice. MFIs are normalized to the average MFI of T_reg cells from Csnk2b^fl/fl for each experiment (n = 6 mice per group pooled from 2 independent experiments). Data represent mean +/- SD. (b) Percentage of CD4⁺Foxp3⁺ T_reg cells from Csnk2b^fl/fl and Csnk2b^fl/flFoxp3-Cre mice expressing the indicated Molecules involved in T_reg cell-mediated suppression (CTLA-4 (surface expression and intracellular expression), Granzyme B, CD39 and CD73). Gating was performed according to isotype control staining (n = 6 mice per group pooled from 2 independent experiments). Data represent mean +/- SD. (c) Percentage of CD4⁺Foxp3⁺ T_reg cells from Csnk2b^fl/fl and Csnk2b^fl/flFoxp3-Cre mice expressing components of the IL2-receptor complex (CD25, CD122, common γ chain (γc)). (n = 6 mice per group from 2 independent experiments). Data represent mean +/- SD. (d) Naïve CD4⁺ T cells (T_eff) from C57BL/6 mice were labeled with CFSE and stimulated in coculture with unlabeled Csnk2b-deficient (Csnk2b^-/-) or Csnk2b-sufficient (Csnk2b^+/+) T_reg cells in indicated ratios, as outlined in online methods. On day 4, CFSE fluorescence was measured by FACS analyses. Representatives of three independent experiments are shown.