Supplementary Figure 8: ILT3⁺ T_reg cells are able to suppress T_H1 responses.

Analysis of IFN-γ-producing CD4⁺CD45.1⁺ T cells stimulated in vitro under T_H1-polarizing conditions in the absence (T_eff) or presence of different numbers of either Csnk2b-competent (Csnk2b^+/+) or Csnk2b-deficient (Csnk2b^-/-) T_reg cells (a) and ILT3^- or ILT3⁺ T_reg cells from C57BL/6 wildtype mice (b). For each experiment the amount of IFN-γ producers in absence of T_reg cells (T_eff) was set to 100% and the percentages of IFN-γ-producing T_H1 cells in co-cultures were calculated accordingly. Data show mean +/- SD combined from 4 independent experiments (a) and 3 independent experiments (b). (c) Flow cytometric analysis of T-bet expression in CD4⁺CD44⁺CD45.1⁺ T cells in mesenteric lymph nodes of colitis-bearing mice. Given is the percentage of T-bet⁺ T cells among CD4⁺CD44⁺CD45.1⁺ T cells (n = 4 for T_eff, n = 6 for ILT3^- T_reg cells and n = 7 for ILT3⁺ T_reg cells from one single experiment). Data represent mean +/- SD. (d) Bodyweight changes upon adoptive transfer of CD4⁺CD62L⁺ T_eff in absence or presence of ILT3^-, ILT3⁺ T_reg cells isolated from C57BL/6 wildtype or T_reg cells isolated from either Csnk2b^fl/flFoxp3-Cre or Csnk2b^fl/fl mice into lymphopenic Rag1^-/- mice as described in online methods. (n = 18 for T_eff, n = 14 for ILT3^- T_reg cells and ILT3⁺ T_reg cells, n = 5 for Csnk2b^-/- T_reg cells and n = 7 for Csnk2b^+/+ T_reg cells from 2 independent experiments. * = p =0.0212; ** = p = 0.0181; *** = p = 0.0101.