Supplementary Figure 8: TAB3 is critical to the PtpA-mediated suppression of cytokine production.
From: Mycobacterium tuberculosis suppresses innate immunity by coopting the host ubiquitin system
(a) TAB3 siRNA-2 could efficiently reduce the expression of TAB3 in both HEK293T and U937 cells as shown by immunoblot analysis. (b) Quantitative PCR analysis showed that TAB3 knockdown abolishes the PtpA-mediated down-regulation of IL1B mRNA expression in U937 cells infected with the indicated mycobacterial strains for 0–48 h. (c,d) ELISA showed that TAB3 knockdown abolishes the PtpA-mediated reduction in TNF protein (c) and IL-1β (d) released by U937 cells infected as in (b). (e) Model mechanisms by which Mtb PtpA modulates innate immune signaling. Quantitative PCR results are presented relative to expression of the control gene Gapdh (in b). *P < 0.05 and **P < 0.01 (two-tailed unpaired t-test). Data are representative of one experiment with at least three independent biological replicates (b–d; mean and s.e.m., n = 3).
