Supplementary Figure 3: IL-1 receptor deficiency alters the balance of Foxp3- and IL-17-expressing T cells during enteropathogenic bacterial infection.

(a) WT B6 mice were inoculated with C. rodentium (2 x 10⁹ cfu) and assessed for frequencies of CD4⁺Foxp3⁺ lymphocytes in MLN and LP at indicated time-points post-infection by flow cytometry (CD4 T cell gate). (b) Pooled data from a showing frequencies of CD4⁺Foxp3⁺ lymphocytes in MLN and colonic LP at indicated time-points post-infection. (c) Il1r1^+/+ Il17f ^Thy-1.1.Foxp3^gfp and Il1r1^–/– Il17f ^Thy-1.1.Foxp3^gfp mice were inoculated with 2 x 10⁹ cfu C. rodentium and analyzed without restimulation ex vivo for frequencies of IL-17F (Thy1.1⁺) and Foxp3 (GFP⁺) CD4⁺ T cells within the activated pool of T cells isolated from colonic lamina propria (LP) at indicated time-points post-infection. Numbers are percentages of cells in the each quadrant. (d) Pooled data from c showing frequencies of IL-17F (Thy1.1⁺) and Foxp3 (GFP⁺) isolated from colonic LP T cells at indicated time points post-infection. Data are representative of one of two similar experiments (a,c) or two independent experiments with 6 or more mice per group as depicted by individual data points corresponding to one mouse (b,d) (means and s.e.m.). *p<0.05, **p<0.01 (two-tailed unpaired T-test). (e) Schematic representation of experiment in Main Fig. 3a,b .