Supplementary Figure 4: In vivo blockade of RA-mediated signaling reduces intestinal bacterial load in the absence of IL-1 signaling. | Nature Immunology

Supplementary Figure 4: In vivo blockade of RA-mediated signaling reduces intestinal bacterial load in the absence of IL-1 signaling.

From: IL-1 signaling modulates activation of STAT transcription factors to antagonize retinoic acid signaling and control the TH17 cell–iTreg cell balance

Supplementary Figure 4

(a) Serial whole-body imaging of Il1r1+/+ Il17f Thy-1.1.Foxp3gfp and Il1r1–/– Il17f Thy-1.1 1.Foxp3gfp mice inoculated with 2 x 109 cfu C. rodentium and gavaged with vehicle alone or with retinoic acid inhibitor (LE135) on days 3-7 post infection and imaged at the indicated days post infection. (b) Colonization kinetics data from a. (c) Body weight kinetics of Il1r1+/+ Il17f Thy-1.1 1.Foxp3gfp and Il1r1–/– Il17f Thy-1.1 1.Foxp3gfp mice inoculated with C. rodentium and gavaged with vehicle alone or with retinoic acid inhibitor (LE135) on indicated days post infection. Data are representative of one of two similar experiments (a) or two independent experiments with 6 mice per group (b, c) (means and s.e.m.). *p<0.05 (Il1r1+/+ vs Il1r1–/–); #p<0.05 (Il1r1–/– + Vehicle vs Il1r1–/– + LE135); **p<0.01 (Il1r1+/+ vs Il1r1–/–) (two-tailed unpaired T-test).

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